Optimal cannabinoid-terpene combination ratios suppress mutagenicity of gastric reflux in normal and metaplastic esophageal cells

Figure 5 Expression of CB1 in human normal esophageal mucosa and esophageal adenocarcinoma assessed by confocal microscopy. A: A representative immunofluorescence analysis of CB1 (red) and epithelial cell adhesion molecule (EPCAM) (green) expression in the normal human esophageal mucosa (magnification 63 ×). The inset highlights the CB1 expression in the basal epithelial layer; B: A representative immunofluorescence analysis depicting CB1 and EPCAM co-expression in the ductal epithelium of the submucosal gland (d) and acini of the submucosal gland (a); C and D: Representative immunofluorescence analyses of CB1 and EPCAM co-expression in two distinct human esophageal adenocarcinoma (EAC) samples; E: Bar graph represents a comparative H-score semiquantitative intensity analysis of CB1 expression in human normal esophageal mucosa and EAC. The data were analyzed using the Mann-Whitney U test. ^bP < 0.01. DAPI: 4’,6-diamidino-2-phenylindole; EPCAM: Epithelial cell adhesion molecule; EAC: Esophageal adenocarcinoma.