Optimal cannabinoid-terpene combination ratios suppress mutagenicity of gastric reflux in normal and metaplastic esophageal cells

Figure 3 Cannabigerol and phytol at a 1:5 ratio reduce deoxycholic acid-induced genomic instability in normal esophageal epithelial cells. A: Scatter plots of the whole genome amplifications and deletions in HET1A cells cultured in the presence of either vehicle control, 100 μmol/L deoxycholic acid (DCA), cannabigerol (CBG)/phytol admixture, or a combination of 100 μmol/L DCA and CBG/phytol admixture for 14 days. DNA extracted at day 14 was compared using precision medicine diversity arrays (Affymetrix) to the DNA of untreated cells collected at day 0; B: Bar graphs illustrate the quantitative analyses of the whole genome amplifications (left panel) and deletions (right panel). Data were analyzed using one-way analysis of variance with multiple comparisons. ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. NS: Not significant; DCA: Deoxycholic acid; CBG: Cannabigerol; Phy: Phytol.