ST2 gene deficiency alleviates acute gastric injury in mice by modulating inflammation and epithelial cell death

Figure 6 Suppression of tumorigenicity 2 deletion downregulates diverse cell death signaling in acute gastric damage. A and B: Real-time quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) analysis of mRNA expression levels of Bcl-2 and Bax (A), and caspase 3 (B) in gastric tissue; C: Percentage of caspase 3 positive cells in the gastric epithelium assessed by immunohistochemical, accompanied by representative images (magnified × 10 and × 40); D: RT-qPCR analysis of mRNA expression levels of Bax/Bcl-2 expression ratio; E and F: The mRNA expression levels of Beclin-1 (E) and high mobility group box-1 (F) gastric tissue detected by RT-qPCR; G: Percentage of tumor necrosis factor-α-positive cells in the gastric epithelium, evaluated by immunohistochemical and illustrated with representative images (magnified × 10 and × 40). Data are expressed as mean ± SEM; n = 10 mice per group. ^aP < 0.05, ^cP < 0.001. WT: Wild type; ST2: Suppression of tumorigenicity 2; HMGB1: High mobility group box-1; TNF-α: Tumor necrosis factor-α.