Role of hepatic sonic hedgehog protein expression in the diagnosis of metabolic dysfunction-associated steatohepatitis

Figure 2 Hematoxylin and eosin, cytokeratin 8/18 and sonic hedgehog stained sections showing ballooned hepatocytes (arrows) in metabolic dysfunction-associated steatohepatitis (200 × magnification). A: Ballooned hepatocytes were enlarged with rarefied cytoplasm; B: Ballooned hepatocytes showed loss of cytokeratin 8/18 expression. Other nonballooned hepatocytes were diffuse positive for cytokeratin 8/18; C: Sonic hedgehog immunostaining highlighted ballooned hepatocytes. Other nonballooned hepatocytes were negative for sonic hedgehog.