Autophagy related RHEB-CSF1R complex promotes tumor metastasis via advancing phosphorylation levels of PI3K, AKT, mTOR in pancreatic cancer

Figure 10  Ras homolog enriched in brain silencing inhibited colony stimulating factor 1 receptor, phosphorylate of phosphatidylinositol 3-kinase, AKT serine/threonine kinase 1, mammalian target of rapamycin, and promoted microtubule-associated protein 1 light chain 3 and E-cadherin in vivo. A and B: Representative images and scores of Ras homolog enriched in brain (RHEB), colony stimulating factor 1 receptor, p-phosphorylate of phosphatidylinositol 3-kinase, p-AKT serine/threonine kinase 1, p-mammalian target of rapamycin. Microtubule-associated protein 1 light chain 3 and E-cadherin immunohistochemical staining in the short hairpin negative control and short hairpin Ras homolog enriched in brain groups. ^aP < 0.05, and ^bP < 0.01. RHEB: Ras homolog enriched in brain; CSF1R: Colony stimulating factor 1 receptor; PI3K: Phosphatidylinositol 3-kinase; AKT: AKT serine/threonine kinase 1; mTOR: Mammalian target of rapamycin; LC3: Microtubule-associated protein 1 light chain 3; shNC: Short hairpin negative control; shRHEB: Short hairpin Ras homolog enriched in brain; IRS: Immunoreactivity score.