Autophagy related RHEB-CSF1R complex promotes tumor metastasis via advancing phosphorylation levels of PI3K, AKT, mTOR in pancreatic cancer

Figure 6 Ras homolog enriched in brain upregulates and interacts with colony stimulating factor 1 receptor in pancreatic cell cells. A-C: The expression of Ras homolog enriched in brain (RHEB) and colony stimulating factor 1 receptor (CSF1R) was confirmed by western blot analyses in the Capan-1 and CFPAC-1 cells transfected with the short hairpin (sh) RNA or the shRHEB, in Colo357 cells transfected with the vector or the RHEB, co-immunoprecipitation assay demonstrated that RHEB directly interacts with CSF1R; D-I: Representative images and quantitative analyses of Transwell assay in the Capan-1 and CFPAC-1 cells stably expressing the small interfering negative control and the small interfering RHEB were transfected transiently with the vector or the CSF1R, Colo357 cells stably expressing vector and RHEB transfected with sh-negative control and shCSF1R. ^aP < 0.05, ^bP < 0.01, and ^cP value < 0.001. RHEB: Ras homolog enriched in brain; shNC: Short hairpin negative control; shRHEB: Short hairpin Ras homolog enriched in brain; CSF1R: Colony stimulating factor 1 receptor; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; siNC: Small interfering negative control; siRHEB: Small interfering Ras homolog enriched in brain; shNC: Short hairpin negative control; shRHEB: Short hairpin Ras homolog enriched in brain.