Autophagy related RHEB-CSF1R complex promotes tumor metastasis via advancing phosphorylation levels of PI3K, AKT, mTOR in pancreatic cancer

Figure 5 Ras homolog enriched in brain promotes proliferation and metastasis in vivo. A-D: Tumor volumes and weights in the short hairpin negative control (shNC) and short hairpin Ras homolog enriched in brain (shRHEB) groups implanted with Capan-1 cells in subcutaneous tumors models; E and F: Representative images and scores of immunohistochemical staining in the shNC and shRHEB groups implanted with Capan-1 cells; G and H: Spleen primary tumors and liver metastases in the shNC and shRHEB groups implanted with Capan-1 cells in liver metastasis models; I: Hematoxylin-eosin staining of harvested tumor. ^aP < 0.05, and ^bP < 0.01. RHEB: Ras homolog enriched in brain; shNC: Short hairpin negative control; shRHEB: Short hairpin Ras homolog enriched in brain; HE: Hematoxylin-eosin staining.