Autophagy related RHEB-CSF1R complex promotes tumor metastasis via advancing phosphorylation levels of PI3K, AKT, mTOR in pancreatic cancer

Figure 4 Ras homolog enriched in brain promotes proliferation and metastasis in pancreatic cell lines. A-C: Measurement of cell proliferation of the Capan-1 and CFPAC-1 cells transfected with the control small interfering RNA (siRNA) or the Ras homolog enriched in brain (RHEB) silencing by siRNA, and in Colo357 cells transfected with the control vector or the RHEB overexpression vector by cell counting kit-8 assay; D-I: Representative images and quantitative analyses of colony formation assay in the Capan-1 and CFPAC-1 cells transfected with the control siRNA or the RHEB silencing by siRNA, and in Colo357 cells transfected with the control vector or the RHEB overexpression vector; J-L: Representative images and quantitative analyses of Transwell assay in the Capan-1 and CFPAC-1 cells transfected with the control siRNA or the RHEB silencing by siRNA, and in Colo357 cells transfected with the control vector or the RHEB overexpression vector. ^aP < 0.05, ^bP < 0.01, and ^cP value < 0.001. RHEB: Ras homolog enriched in brain; CCK-8: Cell counting kit-8; siNC: Small interfering negative control; siRHEB: Small interfering Ras homolog enriched in brain.