Autophagy related RHEB-CSF1R complex promotes tumor metastasis via advancing phosphorylation levels of PI3K, AKT, mTOR in pancreatic cancer

Figure 2 Expression of Ras homolog enriched in brain in pancreatic cancer. A and B: The mRNA expression levels of Ras homolog enriched in brain (RHEB) in five pancreatic cancer (PC) cell lines and normal pancreas line; C: The protein expression levels of RHEB in normal pancreas and PC lines; D: The mRNA expression levels of RHEB in five paired PC and non-tumor tissues; E and F: Scores and representative images of RHEB immunohistochemical staining in the non-paired normal pancreas (n = 15), primary PC (n = 20), distant liver metastasis (n = 18), and lymph node metastasis tissues (n = 6). ^aP < 0.05, ^bP < 0.01, ^cP value < 0.001, and ^dP < 0.0001. PAAD: Pancreatic adenocarcinoma; RT-PCR: Reverse transcription-polymerase chain reaction; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; RHEB: Ras homolog enriched in brain.