Autophagy related RHEB-CSF1R complex promotes tumor metastasis via advancing phosphorylation levels of PI3K, AKT, mTOR in pancreatic cancer

doi:10.3748/wjg.v32.i12.112725

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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Copyright: ©Author(s) 2026. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial (CC BY-NC 4.0) license. No commercial re-use. See permissions. Published by Baishideng Publishing Group Inc.

World J Gastroenterol. Mar 28, 2026; 32(12): 112725
Published online Mar 28, 2026. doi: 10.3748/wjg.v32.i12.112725

Table 2 List of primers used reverse transcription-polymerase chain reaction and reverse transcription-quantitative polymerase chain reaction

Primer	Sequence (5′-3′)	Molecular weight (kDa)	Annealing temperature (°C)
CSF1R-F	ATCTTCTCACTTGGGCTGAATCC	6965.46	60
CSF1R-R	AGATTGGTATAGTCCCGCTCTCT	7005.46
FGF22-F	GTGGTCATCAAAGCAGTGTCCT	6750.27	60
FGF22-R	GTAGGTGTTGTGGCCGTTCT	6185.83
AMTN-F	AGCAGGAGGAGCAGGTGTAAAT	6897.3	60
AMTN-R	ACCAAATTCGAGGCAGCTTAGTT	7047.51
TNFSF12-TNFSF13-F	ATAGCCAGGTCCTGTTTCAAGAC	7023.48	60
TNFSF12-TNFSF13-R	AAATGGAAGACACCTGCGCTATA	7065.63
UQCRHL-F	GGAGGAAGAGGAAGAGGAGGAAT	7324.5	60
UQCRHL-R	CGTGCAACCTCTTCTGTATGTG	6996.45
DACT1-F	AGTGACCTGAGACTGGATGTAGA	7152.49	60
DACT1-R	GGTGCTGGAATGACAACATGGATA	7152.49
ARRDC4-F	ACTGCCATTAGTGATCGGTACAA	7047.51	60
ARRDC-R	CACACTTCTCCCTCACAGTTAGG	6919.45
GAPDH-F	CACTCCTCCACCTTTGACGC	5948.9	60
GAPDH-R	CTGTTGCTGTAGCCAAATTCGT	6716.4

CSF1R: Colony stimulating factor 1 receptor; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.

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Citation: Deng QX, Yang K, He J, Li JF, Li XQ, Zou L, Li YM, Xu SM, Jiang Z, Wu LJ. Autophagy related RHEB-CSF1R complex promotes tumor metastasis via advancing phosphorylation levels of PI3K, AKT, mTOR in pancreatic cancer. World J Gastroenterol 2026; 32(12): 112725
URL: https://www.wjgnet.com/1007-9327/full/v32/i12/112725.htm
DOI: https://dx.doi.org/10.3748/wjg.v32.i12.112725

Deng QX, Yang K, He J, Li JF, Li XQ, Zou L, Li YM, Xu SM, Jiang Z, Wu LJ. Autophagy related RHEB-CSF1R complex promotes tumor metastasis via advancing phosphorylation levels of PI3K, AKT, mTOR in pancreatic cancer. World J Gastroenterol 2026; 32(12): 112725 [DOI: 10.3748/wjg.v32.i12.112725]

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