LIM domain only protein 7 promotes metastasis in colorectal cancer by TGF-β/ZEB1 pathway

Figure 6 Zinc finger E-box binding homeobox 1 regulated the expression of the LMO7 gene in colorectal cancer. A: Prediction analysis revealed that the LIM domain only protein 7 (LMO7) promoter contains zinc finger E-box binding homeobox 1 (ZEB1) transcription binding sites; B: Chromatin immunoprecipitation assay detected ZEB1 binding to the LMO7 promoter by real-time reverse-transcriptase PCR using the indicated LMO7 primers; C: Mutation site in the LMO7 promoter; D: ZEB1 binding to the LMO7 promoter was assayed via dual-luciferase reporter assay; E: ZEB1 and LMO7 expression in ZEB1-overexpressing/knockdown colorectal cancer cells was analyzed by Western blotting. Using the independent samples t-test. ^aP < 0.05. CTL: Cells transfected with the black vector; IgG: Immunoglobulin G; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; LMO7: LIM domain only protein 7; NS: Not significant; shL1: Cells transfected with sh-LMO7-1; shL2: Cells transfected with sh-LMO7-2; ZEBI: Zinc finger E-box binding homeobox 1.