LIM domain only protein 7 promotes metastasis in colorectal cancer by TGF-β/ZEB1 pathway

Figure 3 Overexpression of LIM domain only protein 7 in HCT116 cells enhanced cell proliferation, migration, and invasion. A: The transfection efficiency of HCT116 cells overexpressing LIM domain only protein 7 was detected by real-time reverse-transcriptase PCR; B: The transfection efficiency of HCT116 cells overexpressing LIM domain only protein 7 was detected by western blotting; C: Cell proliferation was detected by a cholecystokinin-8 assay; D: The cell wound scratch assay; E: Statistical analysis; F: The cell invasion experiment; G: Statistical graph. ^aP < 0.05, ^bP < 0.01. Using the independent samples t-test. CTL: Cells transfected with the black vector; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; LMO7: LIM domain only protein 7; LMO7-OV: Cells overexpressing LIM domain only protein 7.