Icariin inhibits tumor-associated neutrophil recruitment via the CXCL8-CXCR2 axis and restrains pro-metastatic programs in colorectal cancer

Figure 5 Icariin affects the biological behavior of colorectal cancer cells via up-regulating SMAD4 expression. A: The mRNA relative expression of SMAD4, C-X-C motif chemokine ligand 1, and C-X-C motif chemokine ligand 8; B: Cell counting kit-8: Cell proliferation; C: Flow cytometry: Apoptosis; D: Transwell: Cell invasion; E: Scratch assay: Cell migration. Results are illustrated as mean ± SD. ^aP < 0.05 vs the small interfering SMAD4-negative control group, ^bP < 0.05 vs the small interfering SMAD4 group, ^cP < 0.05 vs the icariin + small interfering-negative control group. ICA: Icariin; CXCL: C-X-C motif chemokine ligand; siSMAD4: Small interfering SMAD4; NC: Negative control.