Icariin inhibits tumor-associated neutrophil recruitment via the CXCL8-CXCR2 axis and restrains pro-metastatic programs in colorectal cancer

Figure 1 SMAD4-deficient colorectal cancer cells SW480 recruit more C-X-C motif chemokine receptor 2+ tumor-associated neutrophils. A: The relative expression of protein SMAD4, C-X-C motif chemokine ligand 1 (CXCL1), and CXCL8; B: The mRNA relative expression of CXCL1 and CXCL8; C: The proportion of C-X-C motif chemokine receptor 2+ tumor-associated neutrophils in the lower chamber. Results are illustrated as mean ± SD. ^aP < 0.05 vs the normal group, ^bP < 0.05 vs the Caco-2 group. CXCL: C-X-C motif chemokine ligand; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; CXCR: C-X-C motif chemokine receptor.