Chaihu-Shugan-San ameliorates chronic atrophic gastritis by inhibiting nuclear factor-kappa B-mediated inflammation and apoptosis

Figure 6 Effects of Chaihu-Shugan-San on nuclear factor-kappa B signaling in methyl-N’-nitro-N-nitrosoguanidine-treated rats. A: Upper: Western blotting examined the nuclear factor-kappa B signaling proteins inhibitor of nuclear factor-kappa B (IκBα), phospho (p)-IκBα, p65, and p-p65. Lower: Densitometric quantitation of proteins; B: Immunofluorescence detection of expression and nuclear localization of p-p65. Data are shown as mean ± SD (n = 6). Differences were analyzed by one-way analysis of variance followed by Tukey’s post-hoc test. ^bP < 0.01. ^cP < 0.001. P values vs chronic atrophic gastritis model group. NS: No significance; CAG: Chronic atrophic gastritis; CSS: Chaihu-Shugan-San; Con: Control; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; NF-κB: Nuclear factor-kappa B; IκB: Inhibitor of nuclear factor-kappa B; p: Phospho; DAPI: 4’,6-diamidino-2-phenylindole.