Integrative study reveals NR1D1 mediates Hedyotis diffusa’s antifibrosis via hypoxia inducible factor-1/ammonia axis

Figure 4 Hedyotis diffusa injection ameliorates liver fibrosis by influencing circadian rhythm, hypoxia inducible factor-1 signaling pathway and urea metabolic process. A and B: GSE990887 dataset analysis; C and D: GSE33650 dataset analysis; E: Volcano analysis of proteomics in mice; F: Venn analysis of the differentially expressed proteins in these two groups: Control vs carbon tetrachloride (CCl₄) and CCl₄vs Hedyotis diffusa injection; G and H: Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses. KEGG: Kyoto Encyclopedia of Genes and Genomes; TGF: Transforming growth factor; HIF: Hypoxia inducible factor; PPAR: Peroxisome proliferators-activated receptor; PI3K: Phosphatidylinositol 3-kinase; Akt: Protein kinase B; TCA: Tricarboxylic acid cycle; mTOR: Mammalian target of rapamycin; TNF: Tumor necrosis factor; AMPK: Adenosine 5’-monophosphate-activated protein kinase; Th17: T helper 17 cell.