Combined metabolomic and metagenomic analysis reveals inflammatory bowel disease diversity in pediatric and adult patients

Figure 5 Total headspace-gas chromatography-mass spectrometry data obtained for Crohn’s disease group of pediatric and adult patients. A: Principal component analysis and Orthogonal Partial Least Squares Discriminant Analysis data represent two independent groups of samples [Crohn’s disease (CD)-kids, CD-adults]. According to the relative concentration of all detected volatile compounds; B: The nonparametric Mann-Whitney test was used for the primary comparisons between groups. Statistical significance was determined by a two-sided P-value of less than 0.05. False discovery rate correction was also applied; C: Box plots the quantitative differences in the relative contents of metabolites detected in the analyzed groups (CD-kids, CD-adults); D: Risk coefficient calculation. UC: Ulcerative colitis; CD: Crohn’s disease; PCA: Principal component analysis; IBD: Inflammatory bowel disease; OPLS-DA: Orthogonal Partial Least Squares Discriminant Analysis.