IGF2BP3 binds to FBXO32 to activate the cyclic guanosine monophosphate-protein kinase G pathway, promoting gastric cancer progression

Figure 5 FBXO32 is the N⁶-methyladenosine modification target of IGF2BP3. A: Heat map of differentially expressed genes identified by RNA sequencing (RNA-seq); B: Numbers of differentially expressed genes identified by RNA-seq; C: Numbers of N⁶-methyladenosine (m⁶A) peaks identified by methylated RNA immunoprecipitation sequencing (MeRIP-seq); D: M⁶A motifs identified by MeRIP-seq; E: Enriched regions of m⁶A peaks on messenger RNA (mRNA) identified by MeRIP-seq; F: Common genes between the small interfering RNA-negative control group and the small interfering RNA-IGF2BP3 group according to MeRIP-seq; G: Enriched regions of m⁶A peaks on mRNA identified by RNA immunoprecipitation sequencing (RIP-seq); H: Numbers of m⁶A peaks identified by RIP-seq; I: Genes identified by RIP-seq; J: Overlap analysis of genes identified by MeRIP-seq, RIP-seq, and RNA-seq; K: Analysis of the m⁶A site and its abundance on FBXO32 transcripts using Integrative Genomics Viewer software; L: Interaction between IGF2BP3 and FBXO32 as detected by RIP-quantitative polymerase chain reaction; M: Schematic of the wild-type FBXO32 sequence and the m⁶A site-mutated FBXO32 sequence fused to the luciferase reporter vector; N: Relative luciferase activity of wild-type and mutant (site 1427) FBXO32 upon IGF2BP3 overexpression; O: Relative luciferase activity of wild-type and mutant (site 1399) FBXO32 upon IGF2BP3 overexpression; P: Relative luciferase activity of wild-type and mutant (site 1493) FBXO32 upon IGF2BP3 overexpression. Data are shown as the mean ± SD. ^aP < 0.05. ^bP < 0.01. siNC: Small interfering RNA-negative control; siIGF2BP3: Small interfering RNA-IGF2BP3; DEG: Differentially expressed gene; m⁶A: N⁶-methyladenosine; CDS: Coding sequence; UTR: Untranslated regions; RIP-seq: RNA immunoprecipitation sequencing; MeRIP-seq: Methylated RNA immunoprecipitation sequencing; RNA-seq: RNA sequencing; WT: Wild type; Mut: Mutant; NS: Not significant.