IGF2BP3 binds to FBXO32 to activate the cyclic guanosine monophosphate-protein kinase G pathway, promoting gastric cancer progression

Figure 3 Overexpression of IGF2BP3 in gastric cancer cells and human gastric mucosa cells promotes cell proliferation, migration, and invasion in vitro. A and B: Expression of IGF2BP3 messenger RNA in MKN7 and GES-1 cells as detected by quantitative reverse transcription polymerase chain reaction; C and D: Expression of IGF2BP3 protein in MKN7 and GES-1 cells as detected by Western blot; E and F: Effects of IGF2BP3 overexpression on proliferation of MKN7 and GES-1 cells as assessed using cell counting kit-8 assays; G and H: Effects of IGF2BP3 overexpression on migration of MKN7 and GES-1 cells as evaluated through wound healing assays, scale bar: 200 μm; I and J: Effects of IGF2BP3 overexpression on migration of MKN7 and GES-1 cells as determined utilizing transwell migration assays, scale bar: 100 μm; K: Effects of IGF2BP3 overexpression on invasion of MKN7 cells as assessed via transwell invasion assays, scale bar: 100 μm; L and M: Effects of IGF2BP3 overexpression on apoptosis of MKN7 and GES-1 cells as analyzed by flow cytometry. Data are presented as the mean ± SD. ^bP < 0.01. ^cP < 0.001. ^dP < 0.0001. mRNA: Messenger RNA; OD: Optical density.