IGF2BP3 binds to FBXO32 to activate the cyclic guanosine monophosphate-protein kinase G pathway, promoting gastric cancer progression

Figure 2 Knockdown of IGF2BP3 in gastric cancer cells suppresses cell proliferation, migration, and invasion in vitro. A and B: Expression of IGF2BP3 messenger RNA in HGC-27 and MKN74 cells as detected by quantitative reverse transcription polymerase chain reaction; C and D: Expression of IGF2BP3 protein in HGC-27 and MKN74 as detected by Western blot; E and F: Effects of IGF2BP3 knockdown on proliferation of HGC-27 and MKN74 cells as determined by cell counting kit-8 assay; G and H: Effects of IGF2BP3 knockdown on migration of HGC-27 and MKN74 cells as assessed by wound healing assay, scale bar: 200 μm; I and J: Effects of IGF2BP3 knockdown on migration of HGC-27 and MKN74 cells as evaluated using transwell migration assays, scale bar: 100 μm; K and L: Effects of IGF2BP3 knockdown on invasion of HGC-27 and MKN74 cells as assessed using transwell invasion assays, scale bar: 100 μm; M and N: Effects of IGF2BP3 knockdown on apoptosis of HGC-27 and MKN74 cells as determined by flow cytometry. Data are presented as the mean ± SD. ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. ^dP < 0.0001. mRNA: Messenger RNA; siNC: Small interfering RNA-negative control; siIGF2BP3: Small interfering RNA-IGF2BP3; OD: Optical density.