Dialister-driven succinate accumulation is associated with disease activity and postoperative recurrence in Crohn’s disease

Figure 3 Dialister abundance associates with disease activity and postoperative recurrence in Crohn’s disease. A: Classification of individuals into distinct “succinotypes” based on the relative abundances of Dialister and Phascolarctobacterium in Crohn’s disease (CD) and non-inflammatory bowel diseases (IBD) subjects; B: Significant negative correlation between plasma succinate levels and Dialister abundance, a slow succinate-consuming genus (Pearson’s ρ, P value indicated in the figure); C: Comparison of Dialister abundance across active CD, inactive CD, and non-IBD subjects. ^aP < 0.05 vs non-IBD. Statistical analysis: Kruskal-Wallis test followed by pairwise Mann-Whitney U tests; D: Negative correlation between Dialister and Phascolarctobacterium abundances within the cohort (Pearson’s ρ); E: Negative correlation between Phascolarctobacterium abundance and succinate receptor 1 gene expression in peripheral blood mononuclear cells (Pearson’s ρ); F: Mucosal Dialister operational taxonomic unit 750 levels distinguish non-recurrent from recurrent CD patients in uninflamed tissue from the validation cohort; G: Mucosal Dialister (operational taxonomic unit 745) abundance in inflamed mucosa differentiates among non-recurrent, mild-recurrent, and severe recurrent CD patients. Statistical significance was assessed using DESeq2 on non-transformed count data: ^aP < 0.05; ^bP < 0.01; ^cP < 0.001, as indicated in the panels. CD: Crohn’s disease; IBD: Inflammatory bowel diseases; OTU: Operational taxonomic unit; SUCNR1: Succinate receptor 1; NR: Non-recurrent; R: Recurrent; MR: Mild-recurrent; SR: Severe recurrent.