Growth differentiation factor 11 modulates metabolism, mitigating the pro-tumoral behavior provided by M2-like macrophages in hepatocellular carcinoma-derived cells

Figure 6 Effects of the conditioned media from M2-like derived macrophages on the viability and migration of Huh7 cells. A-C: Cell viability was assessed using the Cell Counting Kit-8 kit (24 hours, 48 hours, and 72 hours, respectively), conditioned media from macrophages at different dilutions were used; D: Cell migration assay determined by a wound healing assay, representative images of at least three independent experiments. Scale bars: 300 μm (100 ×, original magnification); E: Plot of the percentage of migration determined by Image J software. Each column represents the mean ± SEM of at least three independent experiments in triplicate. ^aP ≤ 0.05 vs not treated cells. NT: Not treated cells; CM: Conditioned media; FBS: Fetal bovine serum.