Growth differentiation factor 11 modulates metabolism, mitigating the pro-tumoral behavior provided by M2-like macrophages in hepatocellular carcinoma-derived cells

Figure 3 Growth differentiation factor 11 decreases cluster of differentiation 206 in M2-like macrophages. FCM analysis was performed to evaluate cluster of differentiation 206 (CD206) expression. Cells were treated with growth differentiation factor 11 for 72 hours, as stated in the materials and methods section. A: Fluorescence histogram; B: Percentage of CD206⁺ cells; C: Represents the median fluorescence intensity of the subset. Cells were treated with growth differentiation factor 11 for 144 hours, as stated in the materials and methods section; D: Fluorescence histogram; E: Percentage of CD206⁺ events; F: Mean fluorescent intensity in subset. Each column represents the mean ± SEM of at least three independent experiments. ^aP ≤ 0.01 vs M0; ^bP ≤ 0.05 vs post M2-like + growth differentiation factor 11; ^cP ≤ 0.001 vs post M2-like. CD206: Cluster of differentiation 206; GDF11: Growth differentiation factor 11; MFI: Mean fluorescent intensity.