Growth differentiation factor 11 modulates metabolism, mitigating the pro-tumoral behavior provided by M2-like macrophages in hepatocellular carcinoma-derived cells

Figure 2 The anti-inflammatory stimulus acquires M2-like macrophage polarization. A: Fluorescence histogram of the expression of the cluster of differentiation 206 (CD206) marker obtained by flow cytometry; B: Percentage of CD206⁺ cells in IL-4/IL-13-treated macrophages (M2-like); C: Represents the mean fluorescent intensity of the CD206⁺ subset; D: Macrophage morphology under the different treatments, orange arrows show specific morphology. Images are representative of at least three independent experiments. Scale bars: 150 μm (200 ×, original magnification). Each column represents the mean ± SEM of at least three independent experiments. ^aP ≤ 0.05 vs M0; ^bP ≤ 0.05 vs M2-like (24 hours). CD206: Cluster of differentiation 206; MFI: Mean fluorescent intensity; LPS: Lipopolysaccharide; IFN: Interferon; IL: Interleukin.