Lysine acetyltransferase 2A-mediated succinylation of adenosine monophosphate-activated protein kinase suppresses gallstone formation by inhibiting inflammation and pyroptosis

Figure 5 Dorsomorphin treatment increased pyroptosis and inflammation in gallbladder mucosal epithelial cells. A: Western blot was performed to analyze the protein level of phospho-adenosine monophosphate-activated protein kinase (AMPK) (p-AMPK) (T172)/AMPK in gallbladder mucosal epithelial cells (GMECs) (n = 3); B: Quantification of relative protein level of p-AMPK (T172)/AMPK in each group (n = 3); C and D: Flow cytometry was performed to assess the pyroptosis rate in GMECs in each group (n = 3); E and F: Propidium iodide staining was used to analyze cell death in each group (n = 3); G: Enzyme-linked immunosorbent assay was used to measure the concentrations of tumor necrosis factor-α; H: Interleukin (IL)-1β; I: IL-18 in each group (n = 3); J and K: Western blot was performed to assess the protein levels of caspase-1 and gasdermin D-N in each group (n = 3). LPS: Lipopolysaccharide; DOR: Dorsomorphin.