Lysine acetyltransferase 2A-mediated succinylation of adenosine monophosphate-activated protein kinase suppresses gallstone formation by inhibiting inflammation and pyroptosis

Figure 2 Lysine acetyltransferase 2A overexpression suppressed pyroptosis and inflammation in gallbladder mucosal epithelial cells. A: The mRNA expression of lysine acetyltransferase 2A (KAT2A) was analyzed by reverse transcription-quantitative polymerase chain reaction when KAT2A was overexpressed in gallbladder mucosal epithelial cells (GMECs) (n = 3); B and C: Flow cytometry was used to detect the pyroptosis rate in GMECs in each group (n = 3); D and E: Propidium iodide staining was used to analyze cell death in each group (n = 3); F: Enzyme-linked immunosorbent assay was performed to determine the concentrations of tumor necrosis factor-α; G: Interleukin (IL)-1β; H: IL-18 in each group (n = 3); I and J: Western blot was performed to assess the protein levels of caspase-1 and gasdermin D-N in each group (n = 3). LPS: Lipopolysaccharide.