Rutaecarpine targets F-box and WD repeat domain containing 11 to inhibit inflammatory infiltration and alleviate acute pancreatitis

Figure 8 Rutaecarpine modulates F-box and WD repeat domain containing 11 expression through enhancer of zeste homolog 2. The mechanism underlying rutaecarpine’s regulation of F-box and WD repeat domain containing 11 was detected. A: SuperPred prediction of proteins targeted by rutaecarpine (Rut); B: Binding interactions of Rut with enhancer of zeste homolog 2 (EZH2) (molecular docking simulation of Rut binding to EZH2 protein); C: Cellular thermal shift assay verification of Rut binding to EZH2; D: The University of California Santa Cruz database analysis of the FBXW11 promoter; E-J: Reverse transcription-quantitative polymerase chain reaction (E and F) and western blotting (G-J) detection of EZH2 mRNA and protein expression in both animal and cell models of acute pancreatitis treated with Rut; K-M: Effects of EZH2 overexpression and EZH2 knockdown transfection on FBXW11 expression; N and O: Co-immunoprecipitation detection of EZH2-histone H3 binding (N) and H3 methylation (O). n = 3; ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001. EZH2: Enhancer of zeste homolog 2; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; Rut: Rutaecarpine; AP: Acute pancreatitis; FBXW11: F-box and WD repeat domain containing 11; OE-NC: Overexpression negative control; OE-EZH2: Enhancer of zeste homolog 2 overexpression; shNC: Short hairpin RNA negative control; sh-EZH2: Enhancer of zeste homolog 2 knockdown; IgG: Immunoglobulin G; H3: Histone H3; IP: Immunoprecipitation.