Rutaecarpine targets F-box and WD repeat domain containing 11 to inhibit inflammatory infiltration and alleviate acute pancreatitis

Figure 5 F-box and WD repeat domain containing 11 eliminates the protective effect of rutaecarpine on acute pancreatitis. AR42J cells were transfected with F-box and WD repeat domain containing 11 overexpression and then pre-incubated with rutaecarpine before cerulein treatment. A-F: Reverse transcription-quantitative polymerase chain reaction (A and B) and western blotting (C-F) detection of F-box and WD repeat domain containing 11 mRNA and protein in both animal and cell models of acute pancreatitis; G-I: Cell Counting Kit-8 (G) and flow cytometry (H and I) assays to detect cell viability and apoptosis, respectively; J-L: Malondialdehyde, superoxide dismutase, and glutathione levels; M and N: Immunofluorescence detection of reactive oxygen species. n = 3; ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001. FBXW11: F-box and WD repeat domain containing 11; AP: Acute pancreatitis; Rut: Rutaecarpine; OE-NC: Overexpression negative control; OE-FBXW11: F-box and WD repeat domain containing 11 overexpression; PI: Propidium iodide; MDA: Malondialdehyde; SOD: Superoxide dismutase; GSH: Glutathione; ROS: Reactive oxygen species.