Rutaecarpine targets F-box and WD repeat domain containing 11 to inhibit inflammatory infiltration and alleviate acute pancreatitis

Figure 4 Impact of F-box and WD repeat domain containing 11 on acute pancreatitis cells. AR42J cells were transfected with F-box and WD repeat domain containing 11-related vectors before cerulein treatment. A-C: Reverse transcription-quantitative polymerase chain reaction (A) and western blotting (B and C) detection of F-box and WD repeat domain containing 11 mRNA and protein; D-F: Cell Counting Kit-8 (D) and flow cytometry (E and F) assays to detect cell viability and apoptosis, respectively; G-I: Malondialdehyde, superoxide dismutase, and glutathione levels; J and K: Immunofluorescence detection of reactive oxygen species. n = 3; ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001. FBXW11: F-box and WD repeat domain containing 11; OE-NC: Overexpression negative control; OE-FBXW11: F-box and WD repeat domain containing 11 overexpression; shNC: Short hairpin RNA negative control; shFBXW11: F-box and WD repeat domain containing 11 knockdown; PI: Propidium iodide; MDA: Malondialdehyde; SOD: Superoxide dismutase; GSH: Glutathione; ROS: Reactive oxygen species.