Rutaecarpine targets F-box and WD repeat domain containing 11 to inhibit inflammatory infiltration and alleviate acute pancreatitis

Figure 3 Rutaecarpine alleviates cerulein-induced AR42J cell damage. A cell model of acute pancreatitis was established in AR42J cells that were pre-incubated with rutaecarpine. A and B: Cell Counting Kit-8 was used to determine the safe concentration of rutaecarpine (A) and assess the viability of AR42J cells with optimal concentration (B); C and D: Flow cytometry was used to detect cell apoptosis; E-G: Assay kits were utilized to detect the levels of malondialdehyde, superoxide dismutase, and glutathione; H and I: The positive rate of reactive oxygen species was measured through immunofluorescence staining. n = 3; ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001. OD: Optical density; Rut: Rutaecarpine; PI: Propidium iodide; MDA: Malondialdehyde; SOD: Superoxide dismutase; GSH: Glutathione; ROS: Reactive oxygen species.