Peroxiredoxin 1 inhibits tumorigenesis by activating the NLRP3/GSDMD pathway to induce pyroptosis of colorectal cancer cells

Figure 7 Recombinant peroxiredoxin 1 activates the NLRP3/GSDMD pathway to induce pyroptosis of RKO and SW480 cells. A: The expression of GSDMD-FL, GSDMD-NT, NLRP3 and Caspase-1 (p10) in RKO and SW480 cells detected by western blotting under recombinant peroxiredoxin 1 (rPrdx1) stimulation; B: Survival of RKO and SW480 cells detected by MTT under rPrdx1 stimulation; C: LDH release from RKO and SW480 cells induced by rPrdx1; D: IL-1β release from RKO and SW480 cells induced by rPrdx1; E: IL-18 release from RKO and SW480 cells induced by rPrdx1. rPrdx1: 20 μM for 24 hours. MCC950: 10 μM for 24 hours. Ac-FLTD-CMK: 10 μM for 24 hours. All these experiments were repeated three or four times in independent experiments. Data are expressed as the mean ± SD. ^aP < 0.05; NS: No significance; rPrdx1: Recombinant peroxiredoxin 1.