Peroxiredoxin 1 inhibits tumorigenesis by activating the NLRP3/GSDMD pathway to induce pyroptosis of colorectal cancer cells

Figure 6 Recombinant peroxiredoxin 1 induces LDH release and up-regulation of GSDMD-NT in RKO and SW480 cells. A: LDH release from RKO cells induced by different doses of recombinant peroxiredoxin 1 (rPrdx1) stimulation for 24 hours; B: LDH release from SW480 cells induced by different doses of rPrdx1 stimulation for 24 hours; C: GSDMD full-length (FL) expression in RKO, SW480 and HCT-116 cells detected by western blotting; D: GSDMD-FL and GSDMD N-terminal (NT) expression detected by western blotting in RKO cells under 20 μM rPrdx1 stimulation for 24 hours; E: GSDMD-FL and GSDMD-NT expression detected by western blotting in SW480 cells under 20 μM rPrdx1 stimulation for 24 hours. All these experiments were repeated three or four times in independent experiments. Data are expressed as the mean ± SD; ^aP < 0.05 vs control group (without rPrdx1); ^bP < 0.01 vs control group (without rPrdx1); ^cP < 0.001 vs control group (without rPrdx1). rPrdx1: Recombinant peroxiredoxin 1.