Peroxiredoxin 1 inhibits tumorigenesis by activating the NLRP3/GSDMD pathway to induce pyroptosis of colorectal cancer cells

Figure 4 Recombinant peroxiredoxin 1 inhibits proliferation and invasion of RKO and SW480 cells. A: Representative images of proliferation ability detected by the colony formation assay under stimulation by 10 μM or 20 μM recombinant peroxiredoxin 1 (rPrdx1) for 24 hours, scale bar: 1 cm; B: Representative images of invasion ability detected by the Transwell assay with stimulation by 10 μM or 20 μM rPrdx1 for 24 hours, scale bar: 200 μm (200 ×); the experiments for the colony formation assay and Transwell assay were repeated three times in independent experiments; C: Tumor volume analyses showed the inhibitory effect of intravenous injection of rPrdx1 on subcutaneous tumor formation in nude mice (n = 6/group). Bonferroni correction was used to adjust the significance for multiple comparisons after two-way RM ANOVA. Data are expressed as the mean ± SD. ^aP < 0.05 vs control group (without rPrdx1); ^bP < 0.01 vs control group (without rPrdx1). rPrdx1: Recombinant peroxiredoxin 1.