Dopamine receptor D1-mediated suppression of liver fibrosis via Hippo/Yes-associated protein 1 signaling in levodopa treatment

Figure 2 Morphological, histopathological and immunohistochemical analysis of the effect of L-DOPA on carbon tetrachloride-induced liver fibrosis. A: Representative images of rat liver treated with hematoxylin-eosin and Masson's staining, and α-smooth muscle actin (α-SMA) immunohistochemical staining; B: Western blotting analysis of α-SMA in lysed liver tissue; C and D: The results were standardized relative to of GAPDH expression, and the representative protein bands are displayed on the corresponding histogram. ImageJ software (NIH) was used for quantitative analysis of α-SMA positive area, n = 8/group. ^cP < 0.001; NS: Not significant; H&E: Hematoxylin and eosin; α-SMA: Α-smooth muscle actin; CCl₄: Carbon tetrachloride.