Novel chromogenic medium-based method for the rapid detection of Helicobacter pylori drug resistance

doi:10.3748/wjg.v31.i32.106424

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Aug 28, 2025; 31(32): 106424
Published online Aug 28, 2025. doi: 10.3748/wjg.v31.i32.106424

Table 5 Comparison of minimum inhibitory concentrations between Columbia medium and characteristic medium (μg/mL)

*H. pylori*	Columbia medium				Characteristic medium
	LVX	CLR	MTZ	AMX	LVX	CLR	MTZ	AMX
	0.5	0.5	4	0.0625	0.5	0.5	4	0.0625
G27	-	-	-	-	×	×	×	×
26695	-	-	-	-	×	×	×	×
159	+	+	+	+	√	√	√	√
161	+	+	+	+	√	√	√	√
162	+	+	+	+	√	√	√	√
163	+	+	+	+	√	√	√	√
286	+	+	+	+	√	√	√	√
287	+	+	+	+	√	√	√	√
289	+	+	+	+	√	√	√	√
290	+	+	+	+	√	√	√	√

+: Indicates bacterial growth; -: Signifies the absence of bacterial growth; ×: Denotes no bacterial growth and no color change in the medium after 72 hours of incubation. √: Indicates a discoloration observed in the medium after 28-36 hours of incubation. Notably, Helicobacter pylori growth was confirmed after 72 hours of culture. LVX: Levofloxacin; CLR: Clarithromycin; MTZ: Metronidazole; AMX: Amoxicillin; Helicobacter pylori: H. pylori.

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Citation: Guan AX, Yang SY, Wu T, Zhou WT, Chen H, Huang ZS, Luo PP, Huang YQ. Novel chromogenic medium-based method for the rapid detection of Helicobacter pylori drug resistance. World J Gastroenterol 2025; 31(32): 106424
URL: https://www.wjgnet.com/1007-9327/full/v31/i32/106424.htm
DOI: https://dx.doi.org/10.3748/wjg.v31.i32.106424

Guan AX, Yang SY, Wu T, Zhou WT, Chen H, Huang ZS, Luo PP, Huang YQ. Novel chromogenic medium-based method for the rapid detection of Helicobacter pylori drug resistance. World J Gastroenterol 2025; 31(32): 106424 [PMID: 40900768 DOI: 10.3748/wjg.v31.i32.106424]

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