Novel chromogenic medium-based method for the rapid detection of Helicobacter pylori drug resistance

Table 4 Effects of pH value and nickel chloride concentration on the growth of Helicobacter pylori and the discoloration of media

H. pylori	pH			NiCl2 (mmol/L)
	7.15	7.25	7.35	1	10	100
G27	1	1	2	+	+	++
26695	1	1	2	+	+	++
159	1	1	2	+	+	++
161	1	1	2	+	+	++
162	1	1	2	+	+	++
163	1	1	2	+	+	++
286	1	1	1	+	+	++
287	1	1	2	+	+	++
289	1	1	2	+	+	++
290	1	1	2	+	+	++

1: Indicates that the bacterial culture is viable and grows, while the medium shows no significant discoloration after 24 hours; 2: Indicates that the bacterial culture is viable and grows, and the medium exhibits obvious discoloration after 24 hours of incubation; +: Indicates slight discoloration; ++: Signifies obvious discoloration; NiCl₂: Nickel chloride; Helicobacter pylori: H. pylori.