Novel chromogenic medium-based method for the rapid detection of Helicobacter pylori drug resistance

Table 3 Effects of different urea and phenol red concentrations on the growth of Helicobacter pylori

H. pylori	Urea (mg/mL)						Phenol red (mg/100 mL)
	0.6	0.8	0.9	1	1.2	2.4	0.2	0.4	0.8	1.6
G27	++	++	+	+	+	-	2	3	1	1
26695	++	++	+	+	+	-	2	3	1	1
159	++	++	+	+	+	-	2	3	1	1
161	++	++	+	+	+	-	2	3	1	1
162	++	++	+	+	+	-	2	3	1	1
163	++	++	+	+	+	-	2	3	1	1
286	++	++	+	+	+	-	2	3	1	1
287	++	++	+	+	+	-	2	3	1	1
289	++	++	+	+	+	-	2	3	1	1
290	++	++	+	+	+	-	2	3	1	1

1: Indicates that bacteria can grow, with remarkable discoloration recognition, while exhibiting poor sensitivity; 2: Indicates bacterial growth with notable discoloration recognition, while with relatively poor sensitivity; 3: Represents bacterial growth with excellent discoloration and high sensitivity, for the discoloration and sensitivity assessments; +: Indicates that bacteria can grow, while their growth is affected to a certain extent; ++: Refers to the medium with urea added to the culture of Helicobacter pylori (1 × 10⁵ CFUs), where bacterial growth is not affected or has little impact; -: Signifies that bacteria are affected and do not grow. Helicobacter pylori: H. pylori.