Sex based relative expression of estrogen receptors and tumor necrosis factor-alpha in liver affects hepatitis C virus viral pathogenesis

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World Journal of Gastroenterology

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1007-9327

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Basic Study

World J Gastroenterol. Aug 28, 2025; 31(32): 104277
Published online Aug 28, 2025. doi: 10.3748/wjg.v31.i32.104277

Table 1 Antibodies and positive controls used for western blot

Antibody	Catalog	Host/class/isotype	Concentration	Observed size (kDa) in human liver
Anti-ERβ	Abcam; ab3576	Rabbit/polyclonal/IgG	1:1000; (1:30000 2^nd)	70
Anti-TNF-α	Abcam; ab1793	Mouse/monoclonal/IgG1	1:1000; (1:10000 2^nd)	45
Anti-ERα	Invitrogen; MA5-13191	Mouse/monoclonal/IgG1	1:500; (1:10000 2^nd)	37
Anti-Histone H3	Abcam; ab1791	Rabbit/polyclonal/IgG	1:1000; (1:20000 2^nd)	15
Anti-GAPDH (0411)	Santa Cruz; sc-47724	Mouse/monoclonal/IgG1	1:1000; (1:10000 2^nd)	50
Anti-rabbit IRDye^® 800CW	LI-COR; 926-32213	Donkey/IgG	Varies
Anti-mouse IRDye^® 800CW	LI-COR; 926-32212	Donkey/IgG	Varies
Recombinant human TNF-α	Abcam; ab55237		10 ng/well	25

TNF-α: Tumor necrosis factor-alpha; ER: Estrogen receptor; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Citation: Groover S, Addison S, Nicks S, Mwangi M, Brooks A, Kaul A, Kaul R. Sex based relative expression of estrogen receptors and tumor necrosis factor-alpha in liver affects hepatitis C virus viral pathogenesis. World J Gastroenterol 2025; 31(32): 104277
URL: https://www.wjgnet.com/1007-9327/full/v31/i32/104277.htm
DOI: https://dx.doi.org/10.3748/wjg.v31.i32.104277

Groover S, Addison S, Nicks S, Mwangi M, Brooks A, Kaul A, Kaul R. Sex based relative expression of estrogen receptors and tumor necrosis factor-alpha in liver affects hepatitis C virus viral pathogenesis. World J Gastroenterol 2025; 31(32): 104277 [PMID: 40900774 DOI: 10.3748/wjg.v31.i32.104277]

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