Translocator protein facilitates neutrophil-mediated mucosal inflammation in inflammatory bowel diseases

Figure 3 Expression of translocator protein in the neutrophils of patients with inflammatory bowel diseases. A: Peripheral blood samples are collected from 6 healthy controls (HC), and various immune cell types are isolated using magnetic bead sorting. Translocator protein (TSPO) expression is assessed via quantitative real-time polymerase chain reaction (qRT-PCR); B: Peripheral blood samples are collected from HC (n = 17), patients with active Crohn's disease (A-CD) (n = 19), those with Crohn's disease (CD) in remission (R-CD) (n = 11), those with active ulcerative colitis (A-UC) (n = 12), and those with ulcerative colitis (UC) in remission (R-UC) (n = 12). Neutrophils are isolated from these samples using immunomagnetic beads, and TSPO expression is analyzed using qRT-PCR in each group; C and D: Peripheral blood samples from HC, patients with A-CD, those in R-CD, those with A-UC, and those in R-UC are collected (n = 8 per group). Neutrophils are isolated using immunomagnetic beads, and TSPO expression is measured by flow cytometry; E: Immunofluorescence staining is performed to evaluate TSPO expression in neutrophils present in the intestinal mucosa of HC and patients with active inflammatory bowel diseases (CD and UC). Myeloperoxidase (MPO): Red; TSPO: Green; MPO⁺TSPO⁺: Yellow. Original magnification 400 ×. n: The biologically independent replicates used in the statistical analyses. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. A-CD: Active Crohn's disease; A-UC: Active ulcerative colitis; CD: Crohn's disease; CD-inflamed: Intestinal mucosa from inflammatory sites in patients with Crohn's disease; HC: Healthy controls; Healthy control: Intestinal mucosa from healthy individuals; MFI: Measured by flow cytometry; MPO: Myeloperoxidase; R-CD: Crohn's disease in remission; R-UC: Ulcerative colitis in remission; TSPO: Translocator protein; UC: Ulcerative colitis; UC-inflamed: Intestinal mucosa from inflammatory sites in patients with ulcerative colitis.