Leucine-rich repeat-containing protein 19 suppresses colorectal cancer by targeting cyclin-dependent kinase 6/E2F1 and remodeling the immune microenvironment

Figure 8 Leucine-rich repeat-containing 19 induces G0/G1 cell cycle arrest by regulating cyclin-dependent kinase 6 expression. A and B: Cell cycle distribution analysis via flow cytometry in leucine-rich repeat-containing 19 (LRRC19)-overexpressing HCT116 and HT29 cells; C: Western blot analysis evaluating protein levels of cell cycle regulators (cyclin A2, cyclin B1, cyclin D1, cyclin E2, cyclin-dependent kinase 2 (CDK2), CDK4, and CDK6); glyceraldehyde-3-phosphate dehydrogenase (GAPDH) used as a loading control; D: Validation of CDK6 overexpression in HCT116 (LRRC19) cells transfected with pEGFP-CDK6 plasmids via Western blotting; E and F: Anchorage-independent growth assays demonstrating effects of CDK6 rescue in LRRC19-overexpressing cells; G: Flow cytometry analysis assessing cell cycle progression in CDK6-rescued LRRC19-overexpressing HCT116 cells. Data are presented as the mean ± SD. ^bP < 0.05 compared with respective controls.