Endothelin A receptor in nociceptors is essential for persistent mechanical pain in a chronic pancreatitis of mouse model

Figure 3 Increased expression of calcitonin gene-related peptide in the dorsal root ganglion and spinal cord of mice with chronic pancreatitis. A and B: Representative images of immunofluorescence staining of calcitonin gene-related peptide (CGRP) on the T8-12 dorsal root ganglion (DRG) neurons (A) and spinal cord (B); C: Percentage of CGRP-positive DRG neurons; D: Relative CGRP-positive fiber density (normalized to wild type-vehicle group) in the dorsal spinal cord was analyzed for each group of mice. Scale bar, 50 μm (A) and 100 μm (B). n = 5 mice. Data are shown as mean ± SEM. ^bP < 0.01, ^cP < 0.001, statistical comparison were conducted with one-way analysis of variance with Sidak’s post hoc test. CGRP: Calcitonin gene-related peptide; WT: Wild type; CKO: Conditional knockout; DBTC: Dibutyltin dichloride; DRG: Dorsal root ganglion.