N6-methyladenosine reader IGF2BP2 regulates NIPSNAP1-mediated mitophagy and mitochondrial dynamics to alleviate hepatic ischemia-reperfusion injury

Figure 2 4-nitrophenylphosphatase domain and non-neuronal SNAP25-like protein homolog 1 regulates mitophagy in hepatic ischemia-reperfusion. ^aP < 0.05, ^bP < 0.01. A: Hematoxylin eosin staining, n = 6. Scale bar: 100 μm; B and C: Alanine aminotransferase and aspartate aminotransferase activities, n = 6; D: Mice were injected with adeno-associated virus-shRNA-4-nitrophenylphosphatase domain and non-neuronal SNAP25-like protein homolog 1 (NIPSNAP1) and then subjected to hepatic ischemia-reperfusion. NIPSNAP1 protein, n = 3; E: Liver MAP1LC3 II/I and p62 proteins, n = 3; F and G: HepG2 cells were subjected to hypoxia/reoxygenation after transfection with NIPSNAP1 small interfering RNAs or pcDNA-NIPSNAP1. NIPSNAP1, p62 and MAP1LC3 II/I proteins, n = 3; H: Jung’s cyanine dye 1 staining. Scale bar, 50 μm. HE: Hematoxylin eosin; NIPSNAP1: 4-nitrophenylphosphatase domain and non-neuronal SNAP25-like protein homolog 1; AAV: Adeno-associated virus; NC: Negative control; LC3: MAP1LC3; I/R: Ischemia-reperfusion; H/R: Hypoxia/reoxygenation.