N6-methyladenosine reader IGF2BP2 regulates NIPSNAP1-mediated mitophagy and mitochondrial dynamics to alleviate hepatic ischemia-reperfusion injury

Figure 1 4-nitrophenylphosphatase domain and non-neuronal SNAP25-like protein homolog 1 expression in hepatic ischemia-reperfusion. ^aP < 0.05, ^bP < 0.01. A: Liver 4-nitrophenylphosphatase domain and non-neuronal SNAP25-like protein homolog 1 (NIPSNAP1) protein levels at different time points following reperfusion, n = 3; B: NIPSNAP1 mRNA levels, n = 6; C: NIPSNAP1 protein expression in HepG2 cells exposed to hypoxia for 1 hour and after subsequent reoxygenation for 0 hour, 2 hours, 4 hours, 6 hours and 8 hours; n = 3; D: NIPSNAP1 mRNA levels in HepG2 cells subjected to hypoxia/reoxygenation; n = 6; E: Immunofluorescence staining of NIPSNAP1 localized in hepatocytes in the liver sections; scale bars, 20 μm (n = 6). NIPSNAP1: 4-nitrophenylphosphatase domain and non-neuronal SNAP25-like protein homolog 1; H/R: Hypoxia/reoxygenation.