Anti-RPL30 as a novel biomarker for enhanced diagnosis of autoantibody-negative primary biliary cholangitis

Figure 4 ELISA of the autoantibody RPL30 in anti-mitochondrial antibody M2-negative primary biliary cholangitis and healthy control. A: Scatter plot diagram of the optical density value level with the RPL30 antibody in the anti-mitochondrial E2 subunit antibody-negative primary biliary cholangitis group and the healthy control group; B: Analysis of receiver operating characteristic with cutoff value = 0.0708, sensitivity = 90.9%, and specificity = 75%; C: Histogram of RPL30 antibody-positive patients in the anti-mitochondrial E2 subunit antibody-negative primary biliary cholangitis and control groups; D: Analysis of optical density between anti-RPL30, anti-glycoprotein 210, and anti-speckled protein 100 for diagnosis of primary biliary cholangitis. ^aP < 0.001, ^bP < 0.0001. PBC: Primary biliary cholangitis; HC: Healthy control group; OD: Optical density; AUC: Area under the receiver operating characteristic curve; TPR: True positive rate; FPR: False positive rate; M2: Anti-mitochondrial E2 subunit antibody; CI: Confidence interval; Anti-gp210: Anti-glycoprotein 210; Anti-Sp100: Anti-speckled protein 100.