Chaperonin-containing tailless complex polypeptide 1 subunit 6A negatively regulates autophagy and protects colorectal cancer cells from cisplatin-induced cytotoxicity

Figure 5 Chaperonin-containing tailless complex polypeptide 1 subunit 6A negative modulates autophagy induced by cisplatin in colorectal cancer. A-C: Cells were treated with or without cisplatin (DDP) in the presence or absence of CQ for 2 hours, cell lysates were extracted and performed immunoblotting with indicated antibodies; D-G: Following transfection with mCherry-GFP-LC3B adenovirus for 24 hours, cells were split onto coverslips, and then captured by immunofluorescence microscopy (1000 magnification; green arrow: green fluorescence protein (GFP) dot; red arrow: MCherry dot; yellow arrow: Merge dot; white arrowhead: Sole red dot; scale bar = 20 μm). ^aP < 0.05 vs control (Ctrl); CCT6A: Chaperonin-containing tailless complex polypeptide 1 subunit 6a; CQ: Chloroquine diphosphate salt; KO: Knockout; OE: Overexpression.