Chaperonin-containing tailless complex polypeptide 1 subunit 6A negatively regulates autophagy and protects colorectal cancer cells from cisplatin-induced cytotoxicity

Figure 3 Chaperonin-containing tailless complex polypeptide 1 subunit 6a rescues the cell viability loss induced by cisplatin. A and B: The knockout (KO)-chaperonin-containing tailless complex polypeptide 1 subunit 6A (CCT6A) HT29 cells and overexpression (OE)-CCT6A SW480 cells were constructed as described in Methods, and the efficacies were confirmed by immunoblotting and immunofluorescence, respectively. Scale bar = 20 μm; C and D: Utilizing the MTS assay, the cell viability was identified in the indicated cells in the presence or absence of cisplatin (DDP); E-H: Cell proliferation in the indicated cells with or without DDP (μg/mL) treatment was analyzed by colony growth assay (scale bar = 1 cm); I-K: EdU staining assay was performed in the indicated cells with or without DDP treatment (scale bar = 0.5 mm). ^aP < 0.05 vs control (Ctrl); ^bP < 0.01 vs Ctrl.