Strategic insights into the cultivation of pancreatic cancer organoids from endoscopic ultrasonography-guided biopsy tissue

Table 2 Pancreatic puncture sample information and organoid culture information, n (%)

Characteristics	Organoid culture successful (n = 9)	Organoid culture unsuccessful (n = 6)	P value
Sample information
Puncture, 1st needle	8 (89.0)	1 (16.7)	0.005
Sample weight (g), mean ± SD	0.0463 ± 0.0100	0.0387 ± 0.0041	0.494
The time from isolation to organoid culture (hour), mean ± SD	6.2 ± 1.5	18.7 ± 1.5	0.001
Proportion of tumor cells in the sample			0.785
> 70%	0 (0)	0 (0)
20%-70%	3 (33.3)	2 (33.3)
5%-20%	5 (55.6)	4 (66.7)
< 5%	1 (11.1)	0 (0)
Cell activity			0.288
> 50%	0 (0)	0 (0)
15%-50%	5 (55.6)	1 (16.7)
5%-15%	3 (33.3)	5 (83.3)
< 5%	1 (11.1)	0 (0)
Sample quality score, mean ± SD	55.9 ± 2.5	50.2 ± 1.9	0.120
Organoid information
Organoids grow in the first day after processing	8 (88.9)	3 (50.0)	0.107
Organoid proliferation			0.414
Moderate	1 (11.1)	0 (0)
Slow	8 (88.9)	6 (100.0)
Organoid activity			0.031
> 50%	0 (0)	0 (0)
15%-50%	5 (55.6)	0 (0)
5%-15%	4 (44.4)	6 (100.0)
< 5%	0 (0)	0 (0)
Organoid quality score, mean ± SD	64.2 ± 1.6