Chitin-glucan improves important pathophysiological features of irritable bowel syndrome

Figure 6 Modulation of inflammatory-related cytokines in HT-29 cells incubated with chitin-glucan at different concentrations, with or without lipopolysaccharide stimulation. A: Modulation of inflammatory-related cytokines in non-stimulated HT-29 cells incubated for 3 h in medium alone (medium, white), chitin-glucan at 500 microgram per milliliter [chitin glucan (CG): 500, grey)], or chitin-glucan at 1000 microgram per milliliter (CG 1000, dark grey); B: Modulation of inflammatory-related cytokines in HT-29 cells stimulated during 24 h by lipopolysaccharide (LPS) and incubated 3 additional hours in medium alone (LPS, white), chitin-glucan at 500 microgram per milliliter (LPS + CG 500, grey), or chitin-glucan at 1000 microgram per milliliter (LPS + CG 1000, black). ^bP < 0.01. IL-1b: Interleukin-1 beta; IL-8: Interleukin-8; IL-10: Interleukin-10; CG: Chitin glucan; LPS: Lipopolysaccharide.