Fusobacterium nucleatum-induced imbalance in microbiome-derived butyric acid levels promotes the occurrence and development of colorectal cancer

Figure 6 Effects of Fusobacteriumnucleatum and sodium butyrate on mitochondrial membrane potential and reactive oxygen species in colorectal cancer cells. A: Sodium butyrate (NaB) decreased the mitochondrial membrane potential of colorectal cancer cells; however, the mitochondrial membrane potential of the cells recovered after treatment with NaB and Fusobacterium nucleatum supernatant (FAB); B: Statistical results of mitochondrial membrane potential experiments. Data are presented as mean ± standard deviation (SD) from at least three independent experiments; C: NaB stimulates the generation of reactive oxygen species (ROS) in colorectal cancer cells, FAB decreased the effect of NaB and reduced NaB damage to mitochondria; D: Statistical results of ROS. Data from at least three independent experiments are expressed as mean ± SD. ns: P > 0.05, ^aP < 0.05, ^cP < 0.001.