Silent information regulator sirtuin 1 ameliorates acute liver failure via the p53/glutathione peroxidase 4/gasdermin D axis

Figure 7 Silent information regulator sirtuin 1 attenuates hepatocyte ferroptosis and pyroptosis via a p53/glutathione peroxidase 4/gasdermin D dependent mechanism in vitro. A and B: The levels of aspartate aminotransferase and alanine aminotransferase were changed in serum samples of HL7702 cells (n = 3); C and D: The levels of iron and reactive oxygen species were changed in HL7702 cells (n = 3); E: Western blot analyses of silent information regulator sirtuin 1, p53, and acetylated p53 protein expression (n = 3 in each group); F: Western blot analyses of gasdermin D and glutathione peroxidase 4 protein expression (n = 3 in each group); G: Western blot analyses of solute carrier family 7a member 11 and Acyl-CoA synthetase long-chain family member 4 protein expression (n = 3 in each group). ^aP < 0.05, ^bP < 0.01. ALF: Acute liver failure; AST: Aspartate aminotransferase; ALT: Alanine aminotransferase; IL: Interleukin; GPX4: Glutathione peroxidase 4; SLC7A11: Solute carrier family 7a member 11; ACSL4: Acyl-CoA synthetase long-chain family member 4; GSDMD: Gasdermin D; SIRT1: Silent information regulator sirtuin 1; LPS: Lipopolysaccharide; D-GalN: D-galactosamine; RSL3: RAS-selective lethal 3; ROS: Reactive oxygen species.